Journal: Journal of Translational Medicine

Article Title: Are morphological criteria sufficient for the identification of circulating tumor cells in renal cancer?

doi: 10.1186/1479-5876-11-214

Figure Lengend Snippet: Summary of the immunocytochemical analysis of CNHCs with antibodies against CD45, CD31, and CAIX

Article Snippet: The filters were incubated with primary antibodies directed against CAIX (rabbit IgG, NB100-417, Novus Biologicals, Littleton, USA; 3.5 μg/ml ), CD31 (mouse IgG1, M0823, Dako, Glostrup, Denmark; 1 μg/ml) or CD45 (mouse IgG1, M0855, Dako, Glostrup, Denmark; 2.4 μg/ml) diluted in Antibody Diluent (Dako, Glostrup, Denmark) for 30 min at RT followed by application of primary antibody enhancer (Dako, Glostrup, Denmark) for 15 min.

Techniques:

Journal: Journal of Translational Medicine

Article Title: Are morphological criteria sufficient for the identification of circulating tumor cells in renal cancer?

doi: 10.1186/1479-5876-11-214

Figure Lengend Snippet: Immunocytochemical analysis of CNHCs with antibodies against the RCC marker CAIX. Clusters of CNHCs cytomorphologically classified as uncertain malignant (−UMF) with cytoplasmic positive staining with antibodies against the RCC marker CAIX (A) . Clusters of CNHC-UMF and -BF without reactivity for CAIX antibodies ( B and C , respectively). A single CNHC-MF with positive cytoplasmic (D) and without staining for CAIX (E) . Single CAIX-negative CNHC-UMF and -BF ( F and G , respectively).

Article Snippet: The filters were incubated with primary antibodies directed against CAIX (rabbit IgG, NB100-417, Novus Biologicals, Littleton, USA; 3.5 μg/ml ), CD31 (mouse IgG1, M0823, Dako, Glostrup, Denmark; 1 μg/ml) or CD45 (mouse IgG1, M0855, Dako, Glostrup, Denmark; 2.4 μg/ml) diluted in Antibody Diluent (Dako, Glostrup, Denmark) for 30 min at RT followed by application of primary antibody enhancer (Dako, Glostrup, Denmark) for 15 min.

Techniques: Marker, Staining

Journal: Theranostics

Article Title: Hypoxia-induced TGFBI maintains glioma stem cells by stabilizing EphA2

doi: 10.7150/thno.95141

Figure Lengend Snippet: TGFBI is associated with the hypoxic microenvironment in human gliomas. (A) FISH staining of TGFBI in human GBM specimens (Ivy gap datasets). Scale bars: 2 mm; enlarged image: 100 μm. (B) Volcano map showing genes highly expressed in hypoxia region (pseudopalisade and microvascular proliferation regions). Each dot represents a gene. (C) Correlation between TGFBI and mRNA expression of hypoxia-related gene in the CCGA-GBM and TGGA-GBM datasets. CC, correlation coefficient; Dot size and color represent the correlation coefficient. (D) IF staining of TGFBI (green) and two hypoxia-associated markers, HIF1α (above, red) and CA9 (bottom, red), in human GBM specimens. Scale bars: 50 μm; enlarged image: 10 μm. (E) IHC staining demonstrating the association between TGFBI and HIF1α proteins in human gliomas. AOD, Average of density; n = 58.

Article Snippet: Primary antibodies: TGFBI (Abclonal, Cat#A11222, for IB, 1:1000; for IP, 5ug; Proteintech, Cat#10188-AP, for IHC, 1:100; for IF, 1:100), CD133 (Affinity, Cat#BF0403, for IF, 1:100), SOX2 (Proteintech, Cat#66411-1-Ig, for IB, 1:1000; Santa Cruz, Cat#365823, for IF, 1:50; for IHC, 1:50), HIF1α (Proteintech, Cat#20960-1-AP, for IB, 1:1000; for IF, 1:50; for IHC 1:50), carbonic anhydrase 9 (CA9, NOVUS, Cat#NB100-417, for IF, 1:50), OLIG2 (Proteintech, Cat#66513-1-Ig, for IB, 1:1000; for IF, 1:200), Ki67 (Proteintech, Cat#27309-1-AP, for IF, 1:200), EphA2 (Abclonal, Cat#A7183, for IB,1:1000; Santa Cruz, Cat#398832, for IF, 1:50; for IHC, 1:50; for IP, 5ug), Akt (Proteintech, Cat#60203-1-Ig, for IB, 1:5000), phospho-Akt (Ser473) (Proteintech, Cat#66444-1-Ig, for IB, 1:2000; Cell Signaling Technology, Cat#4060, for IF, 1:100; for IHC, 1:50), c-MYC (Proteintech, Cat#67447-1-Ig, for IB, 1:5000; for IF,1:100; for IHC, 1:100), phospho-EphA2 (Y588) (Proteintech, Cat#30263-1-AP, for IB, 1:1000), Integrin αV (Proteintech, Cat#27096-1-AP, for IB, 1:1000), Integrin β5 (CST, Cat#3629, for IB, 1:1000), Notch1 (Cell Signaling Technology, Cat#3608, for IB, 1:1000).

Techniques: Staining, Expressing, Immunohistochemistry

Journal: Scientific reports

Article Title: Carbonic anhydrase IV in lizard chemical signals.

doi: 10.1038/s41598-023-41012-9

Figure Lengend Snippet: Figure 5. Immunoblotting of proteins from femoral gland secretions. PVDF membrane was incubated with a rabbit polyclonal antibody against vertebrate CA-IV, followed by an anti-rabbit antibody. The only protein band obtained can be associated to CA-IV. The original western blot image is available as supplementary material (Fig. S3).

Article Snippet: After 1 h incubation in the blocking solution (5% bovine serum albumin, BSA, in Tris-buffered saline, TBS, buffer) and three additional washes with TBS supplemented with 0,1% Tween (TBST), the membrane was incubated overnight at 4 °C with a primary rabbit polyclonal antibody against vertebrate CA-IV (Proteintech), diluted 1:1000 in 1% BSA in TBST.

Techniques: Western Blot, Membrane, Incubation

Journal: Medical Oncology (Northwood, London, England)

Article Title: Expression of HIF-1α and CAIX in nasopharyngeal carcinoma and their correlation with patients’ prognosis

doi: 10.1007/s12032-014-0304-1

Figure Lengend Snippet: Expression of HIF-1α and CAIX by immunohistochemistry in NPC tissues (original magnification ×400). a Nuclear membrane positive staining of HIF-1α in NPC tissue. b Plasmalemma and cytoplasmic positive staining of CAIX in NPC tissue

Article Snippet: Each section was incubated with protein blocker for 15 min at room temperature followed by mouse anti-human HIF-1α antibody(1:100, Novus Biologicals, Colorado, USA) and rabbit anti-human CAIX antibody (1:400, Novus Biologicals, Colorado, USA)incubation at 4 °C overnight.

Techniques: Expressing, Immunohistochemistry, Membrane, Staining

Journal: Medical Oncology (Northwood, London, England)

Article Title: Expression of HIF-1α and CAIX in nasopharyngeal carcinoma and their correlation with patients’ prognosis

doi: 10.1007/s12032-014-0304-1

Figure Lengend Snippet: The Kaplan–Meier curves for NPC patients with both positive and negative expressions of HIF-1α and CAIX: a overall survival, b progression-free survival, c loco-regional relapse-free survival, d distant metastasis-free survival

Article Snippet: Each section was incubated with protein blocker for 15 min at room temperature followed by mouse anti-human HIF-1α antibody(1:100, Novus Biologicals, Colorado, USA) and rabbit anti-human CAIX antibody (1:400, Novus Biologicals, Colorado, USA)incubation at 4 °C overnight.

Techniques: